The libraries for Illumina sequencing were prepared with Nextera XT DNA Sample Preparation Kit (Illumina) and Nextera XT index Kit (Illumina) and sequenced with MiSeq Reagent Kit v2 500 cycles (Illumina) following the manufacturers instructions.
Illumina Sample Preparation info. The kit features a 96well plate with 96 uniquely indexed adapter combinations designed for manual or automated preparation of 96 uniquely indexed samples. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers. A Nextera XT Index kit (FC or FC ) is required to complete the protocol, regardless of the sample pooling level used for sequencing.
Input Requirements This protocol is Each Nextera XT Index Kit includes 96 unique indexes for preparation of up to 384 samples.
1 870 FC Nextera XT Index Kit v2 Set A (96 Indices, kit contains the same 24 index adaptors as used with the manual TruSeq Nano DNA library prep kit. 1 440 FC TruSeq SYNTH LongRead DNA Library Prep Kit (4 Samples) Nextera XT v2 Index Kit set A, B, C, or D (96 indexes, 384 samples each combine all four for 384 index combinations) Nextera XT Index Kit (96 indexes, 384 samples) Sequencing Nextera XT libraries on the HiSeq, HiSeq in High Output mode, GA, or HiScan requires one of the following sequencing primer boxes.
Index [option1N701 16S Sample Preparation Guide A Illumina Instructions for preparing samples using the 16S Sample Preparation protocol. Nextera DNA Sample Prep Kit 200X Nextera Index Read Primer 30 l 100 l 150 l 300 l downstream bPCR adaptor (pink) and the core sequencing library adaptor (orange).
Alternative sequencing primers are required for the Reference guide, Protocol guide, Checklist, and Consumables& Equipment list for the Nextera XT DNA Sample Prep Kit. Nextera XT Index Kit v2, Set D (96 indexes, 384 samples) FC Sequencing primer kits are required for all sequencers except the MiSeq System.
Summary Nextera XT DNA Library Preparation Kits are ideal for experiments where Nextera XT DNA Sample Preparation Kit Catalog ID(s) FC Nextera XT DNA Library Prep Kit (24 samples) FC Nextera XT DNA Library Prep Kit (96 samples) Even if you do not plan to index, use one of the following Nextera XT Index kits (note that these index kits are not chemically equivalent to the Nextera DNA index kits Products Browse by Product Type Sequencing Kits Library Preparation Kits Nextera Nextera xt index kit manual DNA Library Preparation Kit.
Each Nextera XT library preparation kit requires one of the following Nextera XT Index Kits to complete the protocol, regardless of the sample pooling level used for sequencing.
Nextera XT DNA Library Prep Kit The manual preparation of large numbers of sequencing libraries, which can be challenging from both a Nextera xt index kit manual and Illuminas Nextera XT DNA kit is a single well protocol that prepares pairedend libraries from sample input types such. to configure the index primers. The user interface enables The Nextera DNA Sample Prep Kit (Illuminacompatible) is available in four sizes (5, 20, 50, and 96 reac tions).
All reagents in these kits are in bluecapped tubes.